Yeast System
Yeast Vectors 1
Yeast Vectors 2
A System of Shuttle Vectors and Yeast Host Strains Designed for Efficient Manipulation of DNA in Saccharomyces ceratisiae

Yeast Integrative Plasmid Vectors (YIp)
GAL1 and GAL10 (galactose catabolism) selection
TRP1 selection

Prokaryotic System

Promoters
BBa-J23100 (strong)
BBa-J23108 (medium)
BBa-J23109 (weak)
Terminators
BBa-B0015
RBS
Constitutive

Secretion
HlyA Gene Sequence
A novel C-terminal signal sequence targets Escherichia coli haemolysin directly to the medium
Analysis of the haemolysin transport process through the secretion from Escherichia coli of PCM, CAT or beta-galactosidase fused to the Hly C-terminal signal domain
Analysis of the haemolysin secretion system by PhoA-HlyA fusion proteins

Kumamolisin Info
57 kDa (beta-galactosidase: 540 kDa; source)
1701 bp
567 aa​